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A novel mechanism of “metal gel-shift” by histidine-rich Ni(2+)-binding Hpn protein from Helicobacter pylori strain SS1

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research. Migration of protein that does not correlate with formula MW, termed “gel shifting” appears to be common for histidine-rich protei...

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Detalles Bibliográficos
Autores principales: Shelake, Rahul Mahadev, Ito, Yuki, Masumoto, Junya, Morita, Eugene Hayato, Hayashi, Hidenori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312948/
https://www.ncbi.nlm.nih.gov/pubmed/28207866
http://dx.doi.org/10.1371/journal.pone.0172182