Scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium

Site-directed scarless mutagenesis is an essential tool of modern pathogenesis research. We describe an optimized two-step protocol for genome editing in Salmonella enterica serovar Typhimurium to enable multiple sequential mutagenesis steps in a single strain. The system is based on the λ Red recom...

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Detalles Bibliográficos
Autores principales: Hoffmann, Stefanie, Schmidt, Christiane, Walter, Steffi, Bender, Jennifer K., Gerlach, Roman G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315404/
https://www.ncbi.nlm.nih.gov/pubmed/28212413
http://dx.doi.org/10.1371/journal.pone.0172630

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315404/
https://www.ncbi.nlm.nih.gov/pubmed/28212413
http://dx.doi.org/10.1371/journal.pone.0172630

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