Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations

BACKGROUND: Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams to 76.9 mg/L at most). The present study aims to...

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Detalles Bibliográficos
Autores principales: Wu, Zhimeng, Hong, Haofei, Zhao, Xinrui, Wang, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316389/
https://www.ncbi.nlm.nih.gov/pubmed/28261538
http://dx.doi.org/10.1186/s40643-017-0143-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316389/
https://www.ncbi.nlm.nih.gov/pubmed/28261538
http://dx.doi.org/10.1186/s40643-017-0143-y

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