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Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations
BACKGROUND: Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams to 76.9 mg/L at most). The present study aims to...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316389/ https://www.ncbi.nlm.nih.gov/pubmed/28261538 http://dx.doi.org/10.1186/s40643-017-0143-y |