CRISPR/Cpf1-mediated DNA-free plant genome editing

Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hyeran, Kim, Sang-Tae, Ryu, Jahee, Kang, Beum-Chang, Kim, Jin-Soo, Kim, Sang-Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316869/
https://www.ncbi.nlm.nih.gov/pubmed/28205546
http://dx.doi.org/10.1038/ncomms14406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316869/
https://www.ncbi.nlm.nih.gov/pubmed/28205546
http://dx.doi.org/10.1038/ncomms14406

Ejemplares similares