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CRISPR/Cpf1-mediated DNA-free plant genome editing
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of...
Autores principales: | Kim, Hyeran, Kim, Sang-Tae, Ryu, Jahee, Kang, Beum-Chang, Kim, Jin-Soo, Kim, Sang-Gyu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316869/ https://www.ncbi.nlm.nih.gov/pubmed/28205546 http://dx.doi.org/10.1038/ncomms14406 |
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