A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix

SAVSBPM18 is an engineered streptavidin for affinity purification of both biotinylated biomolecules and recombinant proteins tagged with streptavidin binding peptide (SBP) tags. To develop a user-friendly approach for the preparation of the SAVSBPM18-based affinity matrices, a designer fusion protei...

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Detalles Bibliográficos
Autores principales: Wu, Sau-Ching, Wang, Chris, Hansen, Dave, Wong, Sui-Lam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5318860/
https://www.ncbi.nlm.nih.gov/pubmed/28220817
http://dx.doi.org/10.1038/srep42849

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5318860/
https://www.ncbi.nlm.nih.gov/pubmed/28220817
http://dx.doi.org/10.1038/srep42849

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