High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system

BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the f...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Kang, Su, Lingqia, Duan, Xuguo, Liu, Lina, Wu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319110/
https://www.ncbi.nlm.nih.gov/pubmed/28219382
http://dx.doi.org/10.1186/s12934-017-0649-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319110/
https://www.ncbi.nlm.nih.gov/pubmed/28219382
http://dx.doi.org/10.1186/s12934-017-0649-1

Ejemplares similares