Methylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins

Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. In plants, the method is technically challenging due to the low abundance proteins, non-specific bindin...

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Detalles Bibliográficos
Autores principales: Qin, Guochen, Ma, Jun, Chen, Xiaomei, Chu, Zhaoqing, She, Yi-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320500/
https://www.ncbi.nlm.nih.gov/pubmed/28224978
http://dx.doi.org/10.1038/srep42943

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320500/
https://www.ncbi.nlm.nih.gov/pubmed/28224978
http://dx.doi.org/10.1038/srep42943

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