Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization

The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas...

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Detalles Bibliográficos
Autores principales: Senturk, Serif, Shirole, Nitin H., Nowak, Dawid G., Corbo, Vincenzo, Pal, Debjani, Vaughan, Alexander, Tuveson, David A., Trotman, Lloyd C., Kinney, Justin B., Sordella, Raffaella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322564/
https://www.ncbi.nlm.nih.gov/pubmed/28224990
http://dx.doi.org/10.1038/ncomms14370

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322564/
https://www.ncbi.nlm.nih.gov/pubmed/28224990
http://dx.doi.org/10.1038/ncomms14370

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