Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin

Cholesterol partitions into accessible and sequestered pools in cell membranes. Here, we describe a new assay using fluorescently-tagged anthrolysin O, a cholesterol-binding bacterial toxin, to measure accessible cholesterol in human red blood cells (RBCs). Accessible cholesterol levels were stable...

Descripción completa

Detalles Bibliográficos
Autores principales: Chakrabarti, Rima S, Ingham, Sally A, Kozlitina, Julia, Gay, Austin, Cohen, Jonathan C, Radhakrishnan, Arun, Hobbs, Helen H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323040/
https://www.ncbi.nlm.nih.gov/pubmed/28169829
http://dx.doi.org/10.7554/eLife.23355

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323040/
https://www.ncbi.nlm.nih.gov/pubmed/28169829
http://dx.doi.org/10.7554/eLife.23355

Ejemplares similares