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Immunohistochemical correlates of TP53 somatic mutations in cancer

Despite controversy on the correlation between p53 accumulation and TP53 mutational status, ihas long been used as a surrogate method for mutation analysis. The aim of our study was to characterise the IHC expression features of TP53 somatic mutations and define their occurrence in human cancers. A...

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Autores principales: Murnyák, Balázs, Hortobágyi, Tibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323125/
https://www.ncbi.nlm.nih.gov/pubmed/27626311
http://dx.doi.org/10.18632/oncotarget.11912
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author Murnyák, Balázs
Hortobágyi, Tibor
author_facet Murnyák, Balázs
Hortobágyi, Tibor
author_sort Murnyák, Balázs
collection PubMed
description Despite controversy on the correlation between p53 accumulation and TP53 mutational status, ihas long been used as a surrogate method for mutation analysis. The aim of our study was to characterise the IHC expression features of TP53 somatic mutations and define their occurrence in human cancers. A large-scale database analysis was conducted in the IARC TP53 Database (R17); 7878 mutations with IHC features were retrieved representing 60 distinct tumour sites. The majority of the alterations were immunopositive (p <0.001). Sex was known for 4897 mutations showing a female dominance (57.2%) and females carrying negative mutations were significantly younger. TP53 mutations were divided into three IHC groups according to mutation frequency and IHC positivity. Each group had female dominance. Among the IHC groups, significant correlations were observed with age at diagnosis in breast, bladder, liver, haematopoietic system and head & neck cancers. An increased likelihood of false negative IHC associated with rare nonsense mutations was observed in certain tumour sites. Our study demonstrates that p53 immunopositivity largely correlates with TP53 mutational status but expression is absent in certain mutation types.Besides, describing the complex IHC expression of TP53 somatic mutations, our results reveal some caveats for the diagnostic practice.
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spelling pubmed-53231252017-03-23 Immunohistochemical correlates of TP53 somatic mutations in cancer Murnyák, Balázs Hortobágyi, Tibor Oncotarget Research Paper Despite controversy on the correlation between p53 accumulation and TP53 mutational status, ihas long been used as a surrogate method for mutation analysis. The aim of our study was to characterise the IHC expression features of TP53 somatic mutations and define their occurrence in human cancers. A large-scale database analysis was conducted in the IARC TP53 Database (R17); 7878 mutations with IHC features were retrieved representing 60 distinct tumour sites. The majority of the alterations were immunopositive (p <0.001). Sex was known for 4897 mutations showing a female dominance (57.2%) and females carrying negative mutations were significantly younger. TP53 mutations were divided into three IHC groups according to mutation frequency and IHC positivity. Each group had female dominance. Among the IHC groups, significant correlations were observed with age at diagnosis in breast, bladder, liver, haematopoietic system and head & neck cancers. An increased likelihood of false negative IHC associated with rare nonsense mutations was observed in certain tumour sites. Our study demonstrates that p53 immunopositivity largely correlates with TP53 mutational status but expression is absent in certain mutation types.Besides, describing the complex IHC expression of TP53 somatic mutations, our results reveal some caveats for the diagnostic practice. Impact Journals LLC 2016-09-08 /pmc/articles/PMC5323125/ /pubmed/27626311 http://dx.doi.org/10.18632/oncotarget.11912 Text en Copyright: © 2016 Murnyák et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Murnyák, Balázs
Hortobágyi, Tibor
Immunohistochemical correlates of TP53 somatic mutations in cancer
title Immunohistochemical correlates of TP53 somatic mutations in cancer
title_full Immunohistochemical correlates of TP53 somatic mutations in cancer
title_fullStr Immunohistochemical correlates of TP53 somatic mutations in cancer
title_full_unstemmed Immunohistochemical correlates of TP53 somatic mutations in cancer
title_short Immunohistochemical correlates of TP53 somatic mutations in cancer
title_sort immunohistochemical correlates of tp53 somatic mutations in cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323125/
https://www.ncbi.nlm.nih.gov/pubmed/27626311
http://dx.doi.org/10.18632/oncotarget.11912
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