Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions

In vitro display technologies such as mRNA and cDNA display are powerful tools to create and select functional peptides. However, in some cases, efficiency of mRNA-protein fusion is very low, which results in decreased library size and lower chance of successful selection. In this study, to improve...

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Detalles Bibliográficos
Autores principales: Takahashi, Kazuki, Sunohara, Masato, Terai, Takuya, Kumachi, Shigefumi, Nemoto, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2017
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325054/
https://www.ncbi.nlm.nih.gov/pubmed/28275529
http://dx.doi.org/10.2142/biophysico.14.0_23

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325054/
https://www.ncbi.nlm.nih.gov/pubmed/28275529
http://dx.doi.org/10.2142/biophysico.14.0_23

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