Biases in the SMART-DNA library preparation method associated with genomic poly dA/dT sequences

Avoiding biases in next generation sequencing (NGS) library preparation is crucial for obtaining reliable sequencing data. Recently, a new library preparation method has been introduced which has eliminated the need for the ligation step. This method, termed SMART (switching mechanism at the 5′ end...

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Detalles Bibliográficos
Autores principales: Vardi, Oriya, Shamir, Inbal, Javasky, Elisheva, Goren, Alon, Simon, Itamar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325289/
https://www.ncbi.nlm.nih.gov/pubmed/28235101
http://dx.doi.org/10.1371/journal.pone.0172769

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325289/
https://www.ncbi.nlm.nih.gov/pubmed/28235101
http://dx.doi.org/10.1371/journal.pone.0172769

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