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Biases in the SMART-DNA library preparation method associated with genomic poly dA/dT sequences
Avoiding biases in next generation sequencing (NGS) library preparation is crucial for obtaining reliable sequencing data. Recently, a new library preparation method has been introduced which has eliminated the need for the ligation step. This method, termed SMART (switching mechanism at the 5′ end...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325289/ https://www.ncbi.nlm.nih.gov/pubmed/28235101 http://dx.doi.org/10.1371/journal.pone.0172769 |