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Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein

Partitioning defective protein 3 (Par3) can activate the Tiam1/Rac pathway to inhibit invasion and metastasis in many cancers; however, the role of Par3 in lung adenocarcinoma remains unknown. Here we show that Par3 is downregulated in lung adenocarcinoma tissues and is associated with higher rates...

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Autores principales: Tong, Song, Xia, Tian, Fan, Kai, Jiang, Ke, Zhai, Wei, Li, Jing-Song, Wang, Si-Hua, Wang, Jian-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325440/
https://www.ncbi.nlm.nih.gov/pubmed/27588399
http://dx.doi.org/10.18632/oncotarget.11728
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author Tong, Song
Xia, Tian
Fan, Kai
Jiang, Ke
Zhai, Wei
Li, Jing-Song
Wang, Si-Hua
Wang, Jian-Jun
author_facet Tong, Song
Xia, Tian
Fan, Kai
Jiang, Ke
Zhai, Wei
Li, Jing-Song
Wang, Si-Hua
Wang, Jian-Jun
author_sort Tong, Song
collection PubMed
description Partitioning defective protein 3 (Par3) can activate the Tiam1/Rac pathway to inhibit invasion and metastasis in many cancers; however, the role of Par3 in lung adenocarcinoma remains unknown. Here we show that Par3 is downregulated in lung adenocarcinoma tissues and is associated with higher rates of lymph node metastasis and recurrence. Our functional study demonstrated that knock-down of Par3 promoted lung adenocarcinoma cell growth, cell migration, tumor formation, and metastasis, all of which were effectively inhibited when 14-3-3ζ was silenced. We found that Par3 binded with 14-3-3ζ protein and also showed that Par3 abrogated the binding of 14-3-3ζ to Tiam1, which was responsible for Rac1 activation. Knock-down of 14-3-3ζ inhibited Tiam1/Rac-GTP activation and blocked the invasive behavior of cells lacking Par3. These data suggest that loss of Par3 promotes metastatic behavior in lung adenocarcinoma cells through 14-3-3ζ protein.
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spelling pubmed-53254402017-03-23 Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein Tong, Song Xia, Tian Fan, Kai Jiang, Ke Zhai, Wei Li, Jing-Song Wang, Si-Hua Wang, Jian-Jun Oncotarget Research Paper Partitioning defective protein 3 (Par3) can activate the Tiam1/Rac pathway to inhibit invasion and metastasis in many cancers; however, the role of Par3 in lung adenocarcinoma remains unknown. Here we show that Par3 is downregulated in lung adenocarcinoma tissues and is associated with higher rates of lymph node metastasis and recurrence. Our functional study demonstrated that knock-down of Par3 promoted lung adenocarcinoma cell growth, cell migration, tumor formation, and metastasis, all of which were effectively inhibited when 14-3-3ζ was silenced. We found that Par3 binded with 14-3-3ζ protein and also showed that Par3 abrogated the binding of 14-3-3ζ to Tiam1, which was responsible for Rac1 activation. Knock-down of 14-3-3ζ inhibited Tiam1/Rac-GTP activation and blocked the invasive behavior of cells lacking Par3. These data suggest that loss of Par3 promotes metastatic behavior in lung adenocarcinoma cells through 14-3-3ζ protein. Impact Journals LLC 2016-08-31 /pmc/articles/PMC5325440/ /pubmed/27588399 http://dx.doi.org/10.18632/oncotarget.11728 Text en Copyright: © 2016 Tong et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Tong, Song
Xia, Tian
Fan, Kai
Jiang, Ke
Zhai, Wei
Li, Jing-Song
Wang, Si-Hua
Wang, Jian-Jun
Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title_full Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title_fullStr Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title_full_unstemmed Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title_short Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
title_sort loss of par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325440/
https://www.ncbi.nlm.nih.gov/pubmed/27588399
http://dx.doi.org/10.18632/oncotarget.11728
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