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Impact of heat shock step on bacterial transformation efficiency

CaCl(2) treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl(2 )treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL...

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Detalles Bibliográficos
Autores principales: Rahimzadeh, Maral, Sadeghizadeh, Majid, Najafi, Farhood, Arab, Seyed, Mobasheri, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
https://www.ncbi.nlm.nih.gov/pubmed/28261629