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Impact of heat shock step on bacterial transformation efficiency
CaCl(2) treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl(2 )treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/ https://www.ncbi.nlm.nih.gov/pubmed/28261629 |