Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging. We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to E...

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Detalles Bibliográficos
Autores principales: Shen, Yi, Chen, Yingche, Wu, Jiahui, Shaner, Nathan C., Campbell, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328254/
https://www.ncbi.nlm.nih.gov/pubmed/28241009
http://dx.doi.org/10.1371/journal.pone.0171257

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328254/
https://www.ncbi.nlm.nih.gov/pubmed/28241009
http://dx.doi.org/10.1371/journal.pone.0171257

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