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Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity
MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging. We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to E...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328254/ https://www.ncbi.nlm.nih.gov/pubmed/28241009 http://dx.doi.org/10.1371/journal.pone.0171257 |