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Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica

In this study, we have adopted Golden Gate modular cloning strategy to develop a robust and versatile DNA assembly platform for the nonconventional yeast Yarrowia lipolytica. To this end, a broad set of destination vectors and interchangeable building blocks have been constructed. The DNA modules we...

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Detalles Bibliográficos
Autores principales: Celińska, Ewelina, Ledesma‐Amaro, Rodrigo, Larroude, Macarena, Rossignol, Tristan, Pauthenier, Cyrille, Nicaud, Jean‐Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328822/
https://www.ncbi.nlm.nih.gov/pubmed/28217858
http://dx.doi.org/10.1111/1751-7915.12605