PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli

We recently demonstrated that the Escherichia coli ribosome is robust enough to accommodate foreign 16S rRNAs from diverse gamma- and betaproteobacteria bacteria (Kitahara et al., 2012). Therein, we used the common universal primers Bac8f and UN1541r to obtain a nearly full-length gene. However, we...

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Detalles Bibliográficos
Autores principales: Miyazaki, Kentaro, Sato, Mitsuharu, Tsukuda, Miyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329695/
https://www.ncbi.nlm.nih.gov/pubmed/28293553
http://dx.doi.org/10.3389/fbioe.2017.00014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329695/
https://www.ncbi.nlm.nih.gov/pubmed/28293553
http://dx.doi.org/10.3389/fbioe.2017.00014

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