Cargando…
PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli
We recently demonstrated that the Escherichia coli ribosome is robust enough to accommodate foreign 16S rRNAs from diverse gamma- and betaproteobacteria bacteria (Kitahara et al., 2012). Therein, we used the common universal primers Bac8f and UN1541r to obtain a nearly full-length gene. However, we...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329695/ https://www.ncbi.nlm.nih.gov/pubmed/28293553 http://dx.doi.org/10.3389/fbioe.2017.00014 |