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Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues

Adipose tissue is a major site of energy storage and plays a role in regulation of metabolism through release of adipokines. Here we show that mice with a fat-specific knockout of the miRNA-processing enzyme Dicer (ADicerKO), as well as humans with lipodystrophy, have major decreases in circulating...

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Autores principales: Thomou, Thomas, Mori, Marcelo A., Dreyfuss, Jonathan M., Konishi, Masahiro, Sakaguchi, Masaji, Wolfrum, Christian, Rao, Tata Nageswara, Winnay, Jonathon N., Garcia-Martin, Ruben, Grinspoon, Steven K., Gorden, Phillip, Kahn, C. Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330251/
https://www.ncbi.nlm.nih.gov/pubmed/28199304
http://dx.doi.org/10.1038/nature21365
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author Thomou, Thomas
Mori, Marcelo A.
Dreyfuss, Jonathan M.
Konishi, Masahiro
Sakaguchi, Masaji
Wolfrum, Christian
Rao, Tata Nageswara
Winnay, Jonathon N.
Garcia-Martin, Ruben
Grinspoon, Steven K.
Gorden, Phillip
Kahn, C. Ronald
author_facet Thomou, Thomas
Mori, Marcelo A.
Dreyfuss, Jonathan M.
Konishi, Masahiro
Sakaguchi, Masaji
Wolfrum, Christian
Rao, Tata Nageswara
Winnay, Jonathon N.
Garcia-Martin, Ruben
Grinspoon, Steven K.
Gorden, Phillip
Kahn, C. Ronald
author_sort Thomou, Thomas
collection PubMed
description Adipose tissue is a major site of energy storage and plays a role in regulation of metabolism through release of adipokines. Here we show that mice with a fat-specific knockout of the miRNA-processing enzyme Dicer (ADicerKO), as well as humans with lipodystrophy, have major decreases in circulating exosomal miRNAs. Transplantation of white and especially brown adipose tissue (BAT) into ADicerKO mice restores circulating miRNAs associated with an improvement in glucose tolerance and a reduction of hepatic FGF21 mRNA and circulating FGF21. This gene regulation can be mimicked by administration of normal, but not AdicerKO, serum exosomes. Expression of a human-specific miRNA in BAT of one mouse in vivo can also regulate its 3’UTR-reporter in liver of another mouse through serum exosomal transfer. Thus, adipose tissue constitutes a major source of circulating exosomal miRNAs, and these miRNAs can regulate gene expression in distant tissues thereby serving as novel forms of adipokines.
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spelling pubmed-53302512017-08-15 Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues Thomou, Thomas Mori, Marcelo A. Dreyfuss, Jonathan M. Konishi, Masahiro Sakaguchi, Masaji Wolfrum, Christian Rao, Tata Nageswara Winnay, Jonathon N. Garcia-Martin, Ruben Grinspoon, Steven K. Gorden, Phillip Kahn, C. Ronald Nature Article Adipose tissue is a major site of energy storage and plays a role in regulation of metabolism through release of adipokines. Here we show that mice with a fat-specific knockout of the miRNA-processing enzyme Dicer (ADicerKO), as well as humans with lipodystrophy, have major decreases in circulating exosomal miRNAs. Transplantation of white and especially brown adipose tissue (BAT) into ADicerKO mice restores circulating miRNAs associated with an improvement in glucose tolerance and a reduction of hepatic FGF21 mRNA and circulating FGF21. This gene regulation can be mimicked by administration of normal, but not AdicerKO, serum exosomes. Expression of a human-specific miRNA in BAT of one mouse in vivo can also regulate its 3’UTR-reporter in liver of another mouse through serum exosomal transfer. Thus, adipose tissue constitutes a major source of circulating exosomal miRNAs, and these miRNAs can regulate gene expression in distant tissues thereby serving as novel forms of adipokines. 2017-02-15 2017-02-23 /pmc/articles/PMC5330251/ /pubmed/28199304 http://dx.doi.org/10.1038/nature21365 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Thomou, Thomas
Mori, Marcelo A.
Dreyfuss, Jonathan M.
Konishi, Masahiro
Sakaguchi, Masaji
Wolfrum, Christian
Rao, Tata Nageswara
Winnay, Jonathon N.
Garcia-Martin, Ruben
Grinspoon, Steven K.
Gorden, Phillip
Kahn, C. Ronald
Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title_full Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title_fullStr Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title_full_unstemmed Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title_short Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues
title_sort adipose-derived circulating mirnas regulate gene expression in other tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330251/
https://www.ncbi.nlm.nih.gov/pubmed/28199304
http://dx.doi.org/10.1038/nature21365
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