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Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant

BACKGROUND: Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and biomedicine. Overexpression of soluble and active mTG in E. coli has been limite...

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Detalles Bibliográficos
Autores principales: Javitt, Gabe, Ben-Barak-Zelas, Zohar, Jerabek-Willemsen, Moran, Fishman, Ayelet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331659/
https://www.ncbi.nlm.nih.gov/pubmed/28245818
http://dx.doi.org/10.1186/s12896-017-0339-4