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Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant
BACKGROUND: Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and biomedicine. Overexpression of soluble and active mTG in E. coli has been limite...
Autores principales: | Javitt, Gabe, Ben-Barak-Zelas, Zohar, Jerabek-Willemsen, Moran, Fishman, Ayelet |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331659/ https://www.ncbi.nlm.nih.gov/pubmed/28245818 http://dx.doi.org/10.1186/s12896-017-0339-4 |
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