Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR

BACKGROUND: Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses. OBJECTIVES: Establish if mismatches in the primer and p...

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Detalles Bibliográficos
Autores principales: Kamau, Everlyn, Agoti, Charles N., Lewa, Clement S., Oketch, John, Owor, Betty E., Otieno, Grieven P., Bett, Anne, Cane, Patricia A., Nokes, D. James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331890/
https://www.ncbi.nlm.nih.gov/pubmed/28107671
http://dx.doi.org/10.1016/j.jcv.2016.12.011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331890/
https://www.ncbi.nlm.nih.gov/pubmed/28107671
http://dx.doi.org/10.1016/j.jcv.2016.12.011

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