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Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation

High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data...

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Detalles Bibliográficos
Autores principales: Ketteler, Robin, Freeman, Jamie, Ferraro, Francesco, Bata, Nicole, Cutler, Dan F., Kriston-Vizi, Janos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332014/
https://www.ncbi.nlm.nih.gov/pubmed/28248923
http://dx.doi.org/10.1038/sdata.2017.22