Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation

High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data...

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Detalles Bibliográficos
Autores principales: Ketteler, Robin, Freeman, Jamie, Ferraro, Francesco, Bata, Nicole, Cutler, Dan F., Kriston-Vizi, Janos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332014/
https://www.ncbi.nlm.nih.gov/pubmed/28248923
http://dx.doi.org/10.1038/sdata.2017.22
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author Ketteler, Robin
Freeman, Jamie
Ferraro, Francesco
Bata, Nicole
Cutler, Dan F.
Kriston-Vizi, Janos
author_facet Ketteler, Robin
Freeman, Jamie
Ferraro, Francesco
Bata, Nicole
Cutler, Dan F.
Kriston-Vizi, Janos
author_sort Ketteler, Robin
collection PubMed
description High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other organelles. This dataset is suitable for analysis of morphological parameters that are linked to human umbilical vein endothelial cell (HUVEC) biology.
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spelling pubmed-53320142017-03-03 Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation Ketteler, Robin Freeman, Jamie Ferraro, Francesco Bata, Nicole Cutler, Dan F. Kriston-Vizi, Janos Sci Data Data Descriptor High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other organelles. This dataset is suitable for analysis of morphological parameters that are linked to human umbilical vein endothelial cell (HUVEC) biology. Nature Publishing Group 2017-03-01 /pmc/articles/PMC5332014/ /pubmed/28248923 http://dx.doi.org/10.1038/sdata.2017.22 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Ketteler, Robin
Freeman, Jamie
Ferraro, Francesco
Bata, Nicole
Cutler, Dan F.
Kriston-Vizi, Janos
Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title_full Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title_fullStr Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title_full_unstemmed Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title_short Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation
title_sort image-based sirna screen to identify kinases regulating weibel-palade body size control using electroporation
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332014/
https://www.ncbi.nlm.nih.gov/pubmed/28248923
http://dx.doi.org/10.1038/sdata.2017.22
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