Single-cell transcriptome conservation in cryopreserved cells and tissues

A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Guillaumet-Adkins, Amy, Rodríguez-Esteban, Gustavo, Mereu, Elisabetta, Mendez-Lago, Maria, Jaitin, Diego A., Villanueva, Alberto, Vidal, August, Martinez-Marti, Alex, Felip, Enriqueta, Vivancos, Ana, Keren-Shaul, Hadas, Heath, Simon, Gut, Marta, Amit, Ido, Gut, Ivo, Heyn, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333448/
https://www.ncbi.nlm.nih.gov/pubmed/28249587
http://dx.doi.org/10.1186/s13059-017-1171-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333448/
https://www.ncbi.nlm.nih.gov/pubmed/28249587
http://dx.doi.org/10.1186/s13059-017-1171-9

Ejemplares similares