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Single-cell transcriptome conservation in cryopreserved cells and tissues
A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling fr...
Autores principales: | Guillaumet-Adkins, Amy, Rodríguez-Esteban, Gustavo, Mereu, Elisabetta, Mendez-Lago, Maria, Jaitin, Diego A., Villanueva, Alberto, Vidal, August, Martinez-Marti, Alex, Felip, Enriqueta, Vivancos, Ana, Keren-Shaul, Hadas, Heath, Simon, Gut, Marta, Amit, Ido, Gut, Ivo, Heyn, Holger |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333448/ https://www.ncbi.nlm.nih.gov/pubmed/28249587 http://dx.doi.org/10.1186/s13059-017-1171-9 |
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