Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements....

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Detalles Bibliográficos
Autores principales: Pulido-Quetglas, Carlos, Aparicio-Prat, Estel, Arnan, Carme, Polidori, Taisia, Hermoso, Toni, Palumbo, Emilio, Ponomarenko, Julia, Guigo, Roderic, Johnson, Rory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333799/
https://www.ncbi.nlm.nih.gov/pubmed/28253259
http://dx.doi.org/10.1371/journal.pcbi.1005341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333799/
https://www.ncbi.nlm.nih.gov/pubmed/28253259
http://dx.doi.org/10.1371/journal.pcbi.1005341

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