Improving the reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis α-amylase through stabilizing a long loop in domain B

The reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis α-amylase were improved through site-directed mutagenesis. By using multiple sequence alignment and PoPMuSiC algorithm, Ser187 and Asn188, which located within a long loop in Domain B of Bacillus licheniform...

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Detalles Bibliográficos
Autores principales: Li, Zhu, Duan, Xuguo, Chen, Sheng, Wu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333897/
https://www.ncbi.nlm.nih.gov/pubmed/28253342
http://dx.doi.org/10.1371/journal.pone.0173187

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333897/
https://www.ncbi.nlm.nih.gov/pubmed/28253342
http://dx.doi.org/10.1371/journal.pone.0173187

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