Pooled CRISPR screening with single-cell transcriptome read-out

CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such a...

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Detalles Bibliográficos
Autores principales: Datlinger, Paul, Rendeiro, André F, Schmidl, Christian, Krausgruber, Thomas, Traxler, Peter, Klughammer, Johanna, Schuster, Linda C, Kuchler, Amelie, Alpar, Donat, Bock, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334791/
https://www.ncbi.nlm.nih.gov/pubmed/28099430
http://dx.doi.org/10.1038/nmeth.4177

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334791/
https://www.ncbi.nlm.nih.gov/pubmed/28099430
http://dx.doi.org/10.1038/nmeth.4177

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