Total chemical synthesis of a thermostable enzyme capable of polymerase chain reaction

Polymerase chain reaction (PCR) has been a defining tool in modern biology. Towards realizing mirror-image PCR, we have designed and chemically synthesized a mutant version of the 352-residue thermostable Sulfolobus solfataricus P2 DNA polymerase IV with l-amino acids and tested its PCR activity bio...

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Detalles Bibliográficos
Autores principales: Xu, Weiliang, Jiang, Wenjun, Wang, Jiaxing, Yu, Linping, Chen, Ji, Liu, Xianyu, Liu, Lei, Zhu, Ting F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335361/
https://www.ncbi.nlm.nih.gov/pubmed/28265464
http://dx.doi.org/10.1038/celldisc.2017.8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335361/
https://www.ncbi.nlm.nih.gov/pubmed/28265464
http://dx.doi.org/10.1038/celldisc.2017.8

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