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Total chemical synthesis of a thermostable enzyme capable of polymerase chain reaction

Polymerase chain reaction (PCR) has been a defining tool in modern biology. Towards realizing mirror-image PCR, we have designed and chemically synthesized a mutant version of the 352-residue thermostable Sulfolobus solfataricus P2 DNA polymerase IV with l-amino acids and tested its PCR activity bio...

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Detalles Bibliográficos
Autores principales:	Xu, Weiliang, Jiang, Wenjun, Wang, Jiaxing, Yu, Linping, Chen, Ji, Liu, Xianyu, Liu, Lei, Zhu, Ting F
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335361/ https://www.ncbi.nlm.nih.gov/pubmed/28265464 http://dx.doi.org/10.1038/celldisc.2017.8

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