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Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions
Voltage-gated Kv1.3 and Ca(2+)-dependent KCa3.1 are the most prevalent K(+) channels expressed by human and rat T cells. Despite the preferential upregulation of Kv1.3 over KCa3.1 on autoantigen-experienced effector memory T cells, whether Kv1.3 is required for their induction and function is unclea...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337993/ https://www.ncbi.nlm.nih.gov/pubmed/28248292 http://dx.doi.org/10.1038/ncomms14644 |
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author | Chiang, Eugene Y. Li, Tianbo Jeet, Surinder Peng, Ivan Zhang, Juan Lee, Wyne P. DeVoss, Jason Caplazi, Patrick Chen, Jun Warming, Søren Hackos, David H. Mukund, Susmith Koth, Christopher M. Grogan, Jane L. |
author_facet | Chiang, Eugene Y. Li, Tianbo Jeet, Surinder Peng, Ivan Zhang, Juan Lee, Wyne P. DeVoss, Jason Caplazi, Patrick Chen, Jun Warming, Søren Hackos, David H. Mukund, Susmith Koth, Christopher M. Grogan, Jane L. |
author_sort | Chiang, Eugene Y. |
collection | PubMed |
description | Voltage-gated Kv1.3 and Ca(2+)-dependent KCa3.1 are the most prevalent K(+) channels expressed by human and rat T cells. Despite the preferential upregulation of Kv1.3 over KCa3.1 on autoantigen-experienced effector memory T cells, whether Kv1.3 is required for their induction and function is unclear. Here we show, using Kv1.3-deficient rats, that Kv1.3 is involved in the development of chronically activated antigen-specific T cells. Several immune responses are normal in Kv1.3 knockout (KO) rats, suggesting that KCa3.1 can compensate for the absence of Kv1.3 under these specific settings. However, experiments with Kv1.3 KO rats and Kv1.3 siRNA knockdown or channel-specific inhibition of human T cells show that maximal T-cell responses against autoantigen or repeated tetanus toxoid stimulations require both Kv1.3 and KCa3.1. Finally, our data also suggest that T-cell dependency on Kv1.3 or KCa3.1 might be irreversibly modulated by antigen exposure. |
format | Online Article Text |
id | pubmed-5337993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53379932017-03-09 Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions Chiang, Eugene Y. Li, Tianbo Jeet, Surinder Peng, Ivan Zhang, Juan Lee, Wyne P. DeVoss, Jason Caplazi, Patrick Chen, Jun Warming, Søren Hackos, David H. Mukund, Susmith Koth, Christopher M. Grogan, Jane L. Nat Commun Article Voltage-gated Kv1.3 and Ca(2+)-dependent KCa3.1 are the most prevalent K(+) channels expressed by human and rat T cells. Despite the preferential upregulation of Kv1.3 over KCa3.1 on autoantigen-experienced effector memory T cells, whether Kv1.3 is required for their induction and function is unclear. Here we show, using Kv1.3-deficient rats, that Kv1.3 is involved in the development of chronically activated antigen-specific T cells. Several immune responses are normal in Kv1.3 knockout (KO) rats, suggesting that KCa3.1 can compensate for the absence of Kv1.3 under these specific settings. However, experiments with Kv1.3 KO rats and Kv1.3 siRNA knockdown or channel-specific inhibition of human T cells show that maximal T-cell responses against autoantigen or repeated tetanus toxoid stimulations require both Kv1.3 and KCa3.1. Finally, our data also suggest that T-cell dependency on Kv1.3 or KCa3.1 might be irreversibly modulated by antigen exposure. Nature Publishing Group 2017-03-01 /pmc/articles/PMC5337993/ /pubmed/28248292 http://dx.doi.org/10.1038/ncomms14644 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chiang, Eugene Y. Li, Tianbo Jeet, Surinder Peng, Ivan Zhang, Juan Lee, Wyne P. DeVoss, Jason Caplazi, Patrick Chen, Jun Warming, Søren Hackos, David H. Mukund, Susmith Koth, Christopher M. Grogan, Jane L. Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title | Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title_full | Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title_fullStr | Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title_full_unstemmed | Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title_short | Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions |
title_sort | potassium channels kv1.3 and kca3.1 cooperatively and compensatorily regulate antigen-specific memory t cell functions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337993/ https://www.ncbi.nlm.nih.gov/pubmed/28248292 http://dx.doi.org/10.1038/ncomms14644 |
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