An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs

Cryogenic storage of sperm from genetically altered Xenopus improves cost effectiveness and animal welfare associated with their use in research; currently it is routine for X. tropicalis but not reliable for X. laevis. Here we compare directly the three published protocols for Xenopus sperm freeze-...

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Detalles Bibliográficos
Autores principales: Pearl, Esther, Morrow, Sean, Noble, Anna, Lerebours, Adelaide, Horb, Marko, Guille, Matthew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340284/
https://www.ncbi.nlm.nih.gov/pubmed/28237331
http://dx.doi.org/10.1016/j.theriogenology.2017.01.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340284/
https://www.ncbi.nlm.nih.gov/pubmed/28237331
http://dx.doi.org/10.1016/j.theriogenology.2017.01.007

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