Cargando…
An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs
Cryogenic storage of sperm from genetically altered Xenopus improves cost effectiveness and animal welfare associated with their use in research; currently it is routine for X. tropicalis but not reliable for X. laevis. Here we compare directly the three published protocols for Xenopus sperm freeze-...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340284/ https://www.ncbi.nlm.nih.gov/pubmed/28237331 http://dx.doi.org/10.1016/j.theriogenology.2017.01.007 |