Cargando…
New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling
The bio-preservation potential of Lactococcus garvieae lies in its capacity to inhibit the growth of staphylococci, especially Staphylococcus aureus, in dairy products and in vitro. In vitro, inhibition is modulated by the level of aeration, owing to hydrogen peroxide (H(2)O(2)) production by L. gar...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340753/ https://www.ncbi.nlm.nih.gov/pubmed/28337182 http://dx.doi.org/10.3389/fmicb.2017.00359 |
_version_ | 1782512860388130816 |
---|---|
author | Delpech, Pierre Rifa, Etienne Ball, Graham Nidelet, Sabine Dubois, Emeric Gagne, Geneviève Montel, Marie-Christine Delbès, Céline Bornes, Stéphanie |
author_facet | Delpech, Pierre Rifa, Etienne Ball, Graham Nidelet, Sabine Dubois, Emeric Gagne, Geneviève Montel, Marie-Christine Delbès, Céline Bornes, Stéphanie |
author_sort | Delpech, Pierre |
collection | PubMed |
description | The bio-preservation potential of Lactococcus garvieae lies in its capacity to inhibit the growth of staphylococci, especially Staphylococcus aureus, in dairy products and in vitro. In vitro, inhibition is modulated by the level of aeration, owing to hydrogen peroxide (H(2)O(2)) production by L. garvieae under aeration. The S. aureus response to this inhibition has already been studied. However, the molecular mechanisms of L. garvieae underlying the antagonism against S. aureus have never been explored. This study provides evidence of the presence of another extracellular inhibition effector in vitro. This effector was neither a protein, nor a lipid, nor a polysaccharide, nor related to an L-threonine deficiency. To better understand the H(2)O(2)-related inhibition mechanism at the transcriptome level and to identify other mechanisms potentially involved, we used RNA sequencing to determine the transcriptome response of L. garvieae to different aeration levels and to the presence or absence of S. aureus. The L. garvieae transcriptome differed radically between different aeration levels mainly in biological processes related to fundamental functions and nutritional adaptation. The transcriptomic response of L. garvieae to aeration level differed according to the presence or absence of S. aureus. The higher concentration of H(2)O(2) with high aeration was not associated with a higher expression of L. garvieae H(2)O(2)-synthesis genes (pox, sodA, and spxA1) but rather with a repression of L. garvieae H(2)O(2)-degradation genes (trxB1, ahpC, ahpF, and gpx). We showed that L. garvieae displayed an original, previously undiscovered, H(2)O(2) production regulation mechanism among bacteria. In addition to the key factor H(2)O(2), the involvement of another extracellular effector in the antagonism against S. aureus was shown. Future studies should explore the relation between H(2)O(2)-metabolism, H(2)O(2)-producing LAB and the pathogen they inhibit. The nature of the other extracellular effector should also be determined. |
format | Online Article Text |
id | pubmed-5340753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53407532017-03-23 New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling Delpech, Pierre Rifa, Etienne Ball, Graham Nidelet, Sabine Dubois, Emeric Gagne, Geneviève Montel, Marie-Christine Delbès, Céline Bornes, Stéphanie Front Microbiol Microbiology The bio-preservation potential of Lactococcus garvieae lies in its capacity to inhibit the growth of staphylococci, especially Staphylococcus aureus, in dairy products and in vitro. In vitro, inhibition is modulated by the level of aeration, owing to hydrogen peroxide (H(2)O(2)) production by L. garvieae under aeration. The S. aureus response to this inhibition has already been studied. However, the molecular mechanisms of L. garvieae underlying the antagonism against S. aureus have never been explored. This study provides evidence of the presence of another extracellular inhibition effector in vitro. This effector was neither a protein, nor a lipid, nor a polysaccharide, nor related to an L-threonine deficiency. To better understand the H(2)O(2)-related inhibition mechanism at the transcriptome level and to identify other mechanisms potentially involved, we used RNA sequencing to determine the transcriptome response of L. garvieae to different aeration levels and to the presence or absence of S. aureus. The L. garvieae transcriptome differed radically between different aeration levels mainly in biological processes related to fundamental functions and nutritional adaptation. The transcriptomic response of L. garvieae to aeration level differed according to the presence or absence of S. aureus. The higher concentration of H(2)O(2) with high aeration was not associated with a higher expression of L. garvieae H(2)O(2)-synthesis genes (pox, sodA, and spxA1) but rather with a repression of L. garvieae H(2)O(2)-degradation genes (trxB1, ahpC, ahpF, and gpx). We showed that L. garvieae displayed an original, previously undiscovered, H(2)O(2) production regulation mechanism among bacteria. In addition to the key factor H(2)O(2), the involvement of another extracellular effector in the antagonism against S. aureus was shown. Future studies should explore the relation between H(2)O(2)-metabolism, H(2)O(2)-producing LAB and the pathogen they inhibit. The nature of the other extracellular effector should also be determined. Frontiers Media S.A. 2017-03-08 /pmc/articles/PMC5340753/ /pubmed/28337182 http://dx.doi.org/10.3389/fmicb.2017.00359 Text en Copyright © 2017 Delpech, Rifa, Ball, Nidelet, Dubois, Gagne, Montel, Delbès and Bornes. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Delpech, Pierre Rifa, Etienne Ball, Graham Nidelet, Sabine Dubois, Emeric Gagne, Geneviève Montel, Marie-Christine Delbès, Céline Bornes, Stéphanie New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title | New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title_full | New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title_fullStr | New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title_full_unstemmed | New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title_short | New Insights into the Anti-pathogenic Potential of Lactococcus garvieae against Staphylococcus aureus Based on RNA Sequencing Profiling |
title_sort | new insights into the anti-pathogenic potential of lactococcus garvieae against staphylococcus aureus based on rna sequencing profiling |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340753/ https://www.ncbi.nlm.nih.gov/pubmed/28337182 http://dx.doi.org/10.3389/fmicb.2017.00359 |
work_keys_str_mv | AT delpechpierre newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT rifaetienne newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT ballgraham newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT nideletsabine newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT duboisemeric newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT gagnegenevieve newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT montelmariechristine newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT delbesceline newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling AT bornesstephanie newinsightsintotheantipathogenicpotentialoflactococcusgarvieaeagainststaphylococcusaureusbasedonrnasequencingprofiling |