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Hybrid sequencing and map finding (HySeMaFi): optional strategies for extensively deciphering gene splicing and expression in organisms without reference genome

Using second-generation sequencing (SGS) RNA-Seq strategies, extensive alterative splicing prediction is impractical and high variability of isoforms expression quantification is inevitable in organisms without true reference dataset. we report the development of a novel analysis method, termed hybr...

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Detalles Bibliográficos
Autores principales: Ning, Guogui, Cheng, Xu, Luo, Ping, Liang, Fan, Wang, Zhen, Yu, Guoliang, Li, Xin, Wang, Depeng, Bao, Manzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341560/
https://www.ncbi.nlm.nih.gov/pubmed/28272530
http://dx.doi.org/10.1038/srep43793