Harnessing molecular motors for nanoscale pulldown in live cells

Protein–protein interactions (PPIs) regulate assembly of macromolecular complexes, yet remain challenging to study within the native cytoplasm where they normally exert their biological effect. Here we miniaturize the concept of affinity pulldown, a gold-standard in vitro PPI interrogation technique...

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Detalles Bibliográficos
Autores principales: Bird, Jonathan E., Barzik, Melanie, Drummond, Meghan C., Sutton, Daniel C., Goodman, Spencer M., Morozko, Eva L., Cole, Stacey M., Boukhvalova, Alexandra K., Skidmore, Jennifer, Syam, Diana, Wilson, Elizabeth A., Fitzgerald, Tracy, Rehman, Atteeq U., Martin, Donna M., Boger, Erich T., Belyantseva, Inna A., Friedman, Thomas B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341729/
https://www.ncbi.nlm.nih.gov/pubmed/27932498
http://dx.doi.org/10.1091/mbc.E16-08-0583

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341729/
https://www.ncbi.nlm.nih.gov/pubmed/27932498
http://dx.doi.org/10.1091/mbc.E16-08-0583

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