Cargando…

Harnessing molecular motors for nanoscale pulldown in live cells

Protein–protein interactions (PPIs) regulate assembly of macromolecular complexes, yet remain challenging to study within the native cytoplasm where they normally exert their biological effect. Here we miniaturize the concept of affinity pulldown, a gold-standard in vitro PPI interrogation technique...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bird, Jonathan E., Barzik, Melanie, Drummond, Meghan C., Sutton, Daniel C., Goodman, Spencer M., Morozko, Eva L., Cole, Stacey M., Boukhvalova, Alexandra K., Skidmore, Jennifer, Syam, Diana, Wilson, Elizabeth A., Fitzgerald, Tracy, Rehman, Atteeq U., Martin, Donna M., Boger, Erich T., Belyantseva, Inna A., Friedman, Thomas B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The American Society for Cell Biology 2017
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341729/ https://www.ncbi.nlm.nih.gov/pubmed/27932498 http://dx.doi.org/10.1091/mbc.E16-08-0583

Ejemplares similares

Development of Substrate-Selective Probes for Affinity Pulldown of Histone Demethylases
por: Marholz, Laura J., et al.
Publicado: (2014)

Nanobead-based single-molecule pulldown for single cells
por: Zhao, Qirui, et al.
Publicado: (2023)

A smart polymer for sequence-selective binding, pulldown, and release of DNA targets
por: Krieg, Elisha, et al.
Publicado: (2020)

Affinity pulldown of γ-secretase and associated proteins from human and rat brain
por: Teranishi, Yasuhiro, et al.
Publicado: (2010)

Label-Free Single-Molecule Pulldown for the Detection of Released Cellular Protein Complexes
por: Ma, Guangzhong, et al.
Publicado: (2022)

A model of pulldown alignments from SssI-treated DNA improves DNA methylation prediction
por: Moreland, Blythe S., et al.
Publicado: (2019)

Live-cell imaging of actin dynamics reveals mechanisms of stereocilia length regulation in the inner ear
por: Drummond, Meghan C., et al.
Publicado: (2015)

A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
por: Lamont, Elise A., et al.
Publicado: (2014)

Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysates
por: Smith, Robert J., et al.
Publicado: (2023)

Two-color coincidence single-molecule pulldown for the specific detection of disease-associated protein aggregates
por: Saleeb, Rebecca S., et al.
Publicado: (2023)

ILDR1 null mice, a model of human deafness DFNB42, show structural aberrations of tricellular tight junctions and degeneration of auditory hair cells
por: Morozko, Eva L., et al.
Publicado: (2015)

AlphaPulldown—a python package for protein–protein interaction screens using AlphaFold-Multimer
por: Yu, Dingquan, et al.
Publicado: (2022)

Rational Vaccinology: Harnessing Nanoscale Chemical Design for Cancer Immunotherapy
por: Huang, Ziyin, et al.
Publicado: (2022)

Design and use of a back splicing junction probe for pulldown of circular RNA-binding proteins in cell lines
por: Li, Feiya, et al.
Publicado: (2022)

Harnessing mechanical instabilities at the nanoscale to achieve ultra-low stiffness metals
por: Reeve, Samuel Temple, et al.
Publicado: (2017)

Nanobiotics against antimicrobial resistance: harnessing the power of nanoscale materials and technologies
por: Chakraborty, Nayanika, et al.
Publicado: (2022)

Reduction-cleavable desferrioxamine B pulldown system enriches Ni(ii)-superoxide dismutase from a Streptomyces proteome
por: Ni, Jenny, et al.
Publicado: (2023)

Harnessing Multi-Photon Absorption to Produce Three-Dimensional Magnetic Structures at the Nanoscale
por: Hunt, Matthew, et al.
Publicado: (2020)

Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances
por: Pantazis, Antonios, et al.
Publicado: (2018)

Biomimetic nanoscale metal–organic framework harnesses hypoxia for effective cancer radiotherapy and immunotherapy
por: Ni, Kaiyuan, et al.
Publicado: (2020)

Synthesis and structure–activity relationship of peptide nucleic acid probes with improved interstrand-crosslinking abilities: application to biotin-mediated RNA-pulldown
por: Cadoni, Enrico, et al.
Publicado: (2022)

Kinase Inhibitor Pulldown Assay Identifies a Chemotherapy Response Signature in Triple-negative Breast Cancer Based on Purine-binding Proteins
por: Wang, Junkai, et al.
Publicado: (2023)

Harnessing Defect-Tolerance at the Nanoscale: Highly Luminescent Lead Halide Perovskite Nanocrystals in Mesoporous Silica Matrixes
por: Dirin, Dmitry N., et al.
Publicado: (2016)

THE POWER OF REVIEWS: HARNESSING RESEARCH ON COGNITIVE HEALTH
por: Quinn, Kelly, et al.
Publicado: (2019)

The 133-kDa N-terminal domain enables myosin 15 to maintain mechanotransducing stereocilia and is essential for hearing
por: Fang, Qing, et al.
Publicado: (2015)

Harnessing nitric oxide for preventing, limiting and treating the severe pulmonary consequences of COVID-19
por: Adusumilli, Nagasai C., et al.
Publicado: (2020)

Evaluating anticancer properties of Withaferin A—a potent phytochemical
por: Atteeq, Maushma
Publicado: (2022)

Harnessing the Noncanonical Keap1-Nrf2 Pathway for Human Cytomegalovirus Control
por: Ghosh, Ayan K., et al.
Publicado: (2023)

Blended and e-learning in pediatric education: harnessing lessons learned from the COVID-19 pandemic
por: Fitzgerald, Dominic A., et al.
Publicado: (2021)

Harnessing the power of advertising to prevent childhood obesity
por: Bell, Andrew Colin, et al.
Publicado: (2013)

Phenotypic variability of CLDN14 mutations causing DFNB29 hearing loss in the Pakistani population
por: Bashir, Zil-e-Huma, et al.
Publicado: (2012)

Molecular Remodeling of Tip Links Underlies Mechanosensory Regeneration in Auditory Hair Cells
por: Indzhykulian, Artur A., et al.
Publicado: (2013)

Harnessing migraines for neural regeneration
por: Borkum, Jonathan M.
Publicado: (2018)

Harnessing Hibernate
por: Elliott, James, et al.
Publicado: (2008)

Again in Harness
Publicado: (1891)

Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-point accumulation for imaging in nanoscale topography
por: Böger, Carolin, et al.
Publicado: (2019)

Nanoscale Molecular Quantification of Stem Cell–Hydrogel Interactions
por: Maynard, Stephanie A., et al.
Publicado: (2020)

SL-HarDNet: Skin lesion segmentation with HarDNet
por: Bai, Ruifeng, et al.
Publicado: (2023)

Mammalian Auditory Hair Cell Bundle Stiffness Affects Frequency Tuning by Increasing Coupling along the Length of the Cochlea
por: Dewey, James B., et al.
Publicado: (2018)

Harnessing multiple data sources to good program planning for Rural US Veterans
por: Hudson, Teresa, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_ahuiesk6rflduv9d31erjlc606