ITC-derived binding affinity may be biased due to titrant (nano)-aggregation. Binding of halogenated benzotriazoles to the catalytic domain of human protein kinase CK2

The binding of four bromobenzotriazoles to the catalytic subunit of human protein kinase CK2 was assessed by two complementary methods: Microscale Thermophoresis (MST) and Isothermal Titration Calorimetry (ITC). New algorithm proposed for the global analysis of MST pseudo-titration data enabled reli...

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Detalles Bibliográficos
Autores principales: Winiewska, Maria, Bugajska, Ewa, Poznański, Jarosław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342230/
https://www.ncbi.nlm.nih.gov/pubmed/28273138
http://dx.doi.org/10.1371/journal.pone.0173260

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342230/
https://www.ncbi.nlm.nih.gov/pubmed/28273138
http://dx.doi.org/10.1371/journal.pone.0173260

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