Cargando…

Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja

BACKGROUND: Potato frying color is an agronomic trait influenced by the sugar content of tubers. The candidate gene approach was employed to elucidate the molecular basis of this trait in Solanum tuberosum Group Phureja, which is mainly diploid and represents an important genetic resource for potato...

Descripción completa

Detalles Bibliográficos
Autores principales: Duarte-Delgado, Diana, Juyó, Deissy, Gebhardt, Christiane, Sarmiento, Felipe, Mosquera-Vásquez, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345157/
https://www.ncbi.nlm.nih.gov/pubmed/28279167
http://dx.doi.org/10.1186/s12863-017-0489-3
_version_ 1782513662198546432
author Duarte-Delgado, Diana
Juyó, Deissy
Gebhardt, Christiane
Sarmiento, Felipe
Mosquera-Vásquez, Teresa
author_facet Duarte-Delgado, Diana
Juyó, Deissy
Gebhardt, Christiane
Sarmiento, Felipe
Mosquera-Vásquez, Teresa
author_sort Duarte-Delgado, Diana
collection PubMed
description BACKGROUND: Potato frying color is an agronomic trait influenced by the sugar content of tubers. The candidate gene approach was employed to elucidate the molecular basis of this trait in Solanum tuberosum Group Phureja, which is mainly diploid and represents an important genetic resource for potato breeding. The objective of this research was to identify novel genetic variants related with frying quality in loci with key functions in carbohydrate metabolism, with the purpose of discovering genetic variability useful in breeding programs. Therefore, an association analysis was implemented with 109 SNP markers identified in ten candidate genes. RESULTS: The analyses revealed four associations in the locus InvGE coding for an apoplastic invertase and one association in the locus SssI coding for a soluble starch synthase. The SNPs SssI-C (45711901) T and InvGE-C (2475454) T were associated with sucrose content and frying color, respectively, and were not found previously in tetraploid genotypes. The rare haplotype InvGE-A (2475187) C (2475295) A (2475344) was associated with higher fructose contents. Our study allowed a more detailed analysis of the sequence variation of exon 3 from InvGE, which was not possible in previous studies because of the high frequency of insertion-deletion polymorphisms in tetraploid potatoes. CONCLUSION: The association mapping strategy using a candidate gene approach in Group Phureja allowed the identification of novel SNP markers in InvGE and SssI associated with frying color and the tuber sugar content measured by High Performance Liquid Chromatography (HPLC). These novel associations might be useful in potato breeding programs for improving quality traits and to increase crop genetic variability. The results suggest that some genes involved in the natural variation of tuber sugar content and frying color are conserved in both Phureja and tetraploid germplasm. Nevertheless, the associated variants in both types of germplasm were present in different regions of these genes. This study contributes to the understanding of the genetic architecture of tuber sugar contents and frying color at harvest in Group Phureja. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-017-0489-3) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5345157
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-53451572017-03-14 Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja Duarte-Delgado, Diana Juyó, Deissy Gebhardt, Christiane Sarmiento, Felipe Mosquera-Vásquez, Teresa BMC Genet Research Article BACKGROUND: Potato frying color is an agronomic trait influenced by the sugar content of tubers. The candidate gene approach was employed to elucidate the molecular basis of this trait in Solanum tuberosum Group Phureja, which is mainly diploid and represents an important genetic resource for potato breeding. The objective of this research was to identify novel genetic variants related with frying quality in loci with key functions in carbohydrate metabolism, with the purpose of discovering genetic variability useful in breeding programs. Therefore, an association analysis was implemented with 109 SNP markers identified in ten candidate genes. RESULTS: The analyses revealed four associations in the locus InvGE coding for an apoplastic invertase and one association in the locus SssI coding for a soluble starch synthase. The SNPs SssI-C (45711901) T and InvGE-C (2475454) T were associated with sucrose content and frying color, respectively, and were not found previously in tetraploid genotypes. The rare haplotype InvGE-A (2475187) C (2475295) A (2475344) was associated with higher fructose contents. Our study allowed a more detailed analysis of the sequence variation of exon 3 from InvGE, which was not possible in previous studies because of the high frequency of insertion-deletion polymorphisms in tetraploid potatoes. CONCLUSION: The association mapping strategy using a candidate gene approach in Group Phureja allowed the identification of novel SNP markers in InvGE and SssI associated with frying color and the tuber sugar content measured by High Performance Liquid Chromatography (HPLC). These novel associations might be useful in potato breeding programs for improving quality traits and to increase crop genetic variability. The results suggest that some genes involved in the natural variation of tuber sugar content and frying color are conserved in both Phureja and tetraploid germplasm. Nevertheless, the associated variants in both types of germplasm were present in different regions of these genes. This study contributes to the understanding of the genetic architecture of tuber sugar contents and frying color at harvest in Group Phureja. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-017-0489-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-03-09 /pmc/articles/PMC5345157/ /pubmed/28279167 http://dx.doi.org/10.1186/s12863-017-0489-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Duarte-Delgado, Diana
Juyó, Deissy
Gebhardt, Christiane
Sarmiento, Felipe
Mosquera-Vásquez, Teresa
Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title_full Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title_fullStr Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title_full_unstemmed Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title_short Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja
title_sort novel snp markers in invge and sssi genes are associated with natural variation of sugar contents and frying color in solanum tuberosum group phureja
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345157/
https://www.ncbi.nlm.nih.gov/pubmed/28279167
http://dx.doi.org/10.1186/s12863-017-0489-3
work_keys_str_mv AT duartedelgadodiana novelsnpmarkersininvgeandsssigenesareassociatedwithnaturalvariationofsugarcontentsandfryingcolorinsolanumtuberosumgroupphureja
AT juyodeissy novelsnpmarkersininvgeandsssigenesareassociatedwithnaturalvariationofsugarcontentsandfryingcolorinsolanumtuberosumgroupphureja
AT gebhardtchristiane novelsnpmarkersininvgeandsssigenesareassociatedwithnaturalvariationofsugarcontentsandfryingcolorinsolanumtuberosumgroupphureja
AT sarmientofelipe novelsnpmarkersininvgeandsssigenesareassociatedwithnaturalvariationofsugarcontentsandfryingcolorinsolanumtuberosumgroupphureja
AT mosqueravasquezteresa novelsnpmarkersininvgeandsssigenesareassociatedwithnaturalvariationofsugarcontentsandfryingcolorinsolanumtuberosumgroupphureja