Allelic Dropout During Polymerase Chain Reaction due to G-Quadruplex Structures and DNA Methylation Is Widespread at Imprinted Human Loci

Loss of one allele during polymerase chain reaction (PCR) amplification of DNA, known as allelic dropout, can be caused by a variety of mechanisms. Allelic dropout during PCR may have profound implications for molecular diagnostic and research procedures that depend on PCR and assume biallelic ampli...

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Detalles Bibliográficos
Autores principales: Stevens, Aaron J., Taylor, Millie G., Pearce, Frederick Grant, Kennedy, Martin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345703/
https://www.ncbi.nlm.nih.gov/pubmed/28143949
http://dx.doi.org/10.1534/g3.116.038687

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345703/
https://www.ncbi.nlm.nih.gov/pubmed/28143949
http://dx.doi.org/10.1534/g3.116.038687

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