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Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy
Polymorphic variants of the FTO (fat mass and obesity) gene associate with body mass index in humans, but the underlying molecular mechanisms have not been firmly determined. FTO is linked to energy homeostasis via amino acid sensing and is thought to activate the mammalian target of rapamycin compl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347998/ https://www.ncbi.nlm.nih.gov/pubmed/28288181 http://dx.doi.org/10.1371/journal.pone.0168182 |
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author | Aas, Aleksander Isakson, Pauline Bindesbøll, Christian Alemu, Endalkachew A. Klungland, Arne Simonsen, Anne |
author_facet | Aas, Aleksander Isakson, Pauline Bindesbøll, Christian Alemu, Endalkachew A. Klungland, Arne Simonsen, Anne |
author_sort | Aas, Aleksander |
collection | PubMed |
description | Polymorphic variants of the FTO (fat mass and obesity) gene associate with body mass index in humans, but the underlying molecular mechanisms have not been firmly determined. FTO is linked to energy homeostasis via amino acid sensing and is thought to activate the mammalian target of rapamycin complex 1, a negative regulator of autophagy. FTO localises both to the nucleus and the cytoplasm, and in this study we identify a functional nuclear localisation signal (NLS) in the N-terminus of FTO, as well as nuclear localization information in its very C-terminus. Inhibition of FTO nuclear transport has no effect on autophagy and in contrast to a previously proposed role of FTO in autophagy, we find no difference in starvation-induced autophagy in control cells compared to a panel of cell types depleted of FTO. Future studies that further characterise the cellular functions of FTO will be important to understand why variants in FTO are associated with body weight. |
format | Online Article Text |
id | pubmed-5347998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53479982017-03-30 Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy Aas, Aleksander Isakson, Pauline Bindesbøll, Christian Alemu, Endalkachew A. Klungland, Arne Simonsen, Anne PLoS One Research Article Polymorphic variants of the FTO (fat mass and obesity) gene associate with body mass index in humans, but the underlying molecular mechanisms have not been firmly determined. FTO is linked to energy homeostasis via amino acid sensing and is thought to activate the mammalian target of rapamycin complex 1, a negative regulator of autophagy. FTO localises both to the nucleus and the cytoplasm, and in this study we identify a functional nuclear localisation signal (NLS) in the N-terminus of FTO, as well as nuclear localization information in its very C-terminus. Inhibition of FTO nuclear transport has no effect on autophagy and in contrast to a previously proposed role of FTO in autophagy, we find no difference in starvation-induced autophagy in control cells compared to a panel of cell types depleted of FTO. Future studies that further characterise the cellular functions of FTO will be important to understand why variants in FTO are associated with body weight. Public Library of Science 2017-03-13 /pmc/articles/PMC5347998/ /pubmed/28288181 http://dx.doi.org/10.1371/journal.pone.0168182 Text en © 2017 Aas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Aas, Aleksander Isakson, Pauline Bindesbøll, Christian Alemu, Endalkachew A. Klungland, Arne Simonsen, Anne Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title | Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title_full | Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title_fullStr | Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title_full_unstemmed | Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title_short | Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy |
title_sort | nucleocytoplasmic shuttling of fto does not affect starvation-induced autophagy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347998/ https://www.ncbi.nlm.nih.gov/pubmed/28288181 http://dx.doi.org/10.1371/journal.pone.0168182 |
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