Measuring E. coli and bacteriophage DNA in cell sonicates to evaluate the CAL1 reaction as a synthetic biology standard for qPCR

We measured the impact of the presence of total Escherichia coli (E. coli) cellular material on the performance of the Linear Regression of Efficiency (LRE) method of absolute quantitative PCR (LRE qPCR), which features the putatively universal CAL1 calibration reaction, which we propose as a synthe...

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Detalles Bibliográficos
Autores principales: Templar, Alexander, Schofield, Desmond M., Nesbeth, Darren N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348119/
https://www.ncbi.nlm.nih.gov/pubmed/28331815
http://dx.doi.org/10.1016/j.bdq.2016.12.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348119/
https://www.ncbi.nlm.nih.gov/pubmed/28331815
http://dx.doi.org/10.1016/j.bdq.2016.12.001

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