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Development of a Double Nuclear Gene-Targeting Method by Two-Step Transformation Based on a Newly Established Chloramphenicol-Selection System in the Red Alga Cyanidioschyzon merolae

The unicellular red alga Cyanidioschyzon merolae possesses a simple cellular architecture that consists of one mitochondrion, one chloroplast, one peroxisome, one Golgi apparatus, and several lysosomes. The nuclear genome content is also simple, with very little genetic redundancy (16.5 Mbp, 4,775 g...

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Detalles Bibliográficos
Autores principales: Fujiwara, Takayuki, Ohnuma, Mio, Kuroiwa, Tsuneyoshi, Ohbayashi, Ryudo, Hirooka, Shunsuke, Miyagishima, Shin-Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348525/
https://www.ncbi.nlm.nih.gov/pubmed/28352279
http://dx.doi.org/10.3389/fpls.2017.00343