Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

We present an open source high content analysis instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts of fixed or live cells in multiwell plates. This provides a means to screen for cell sig...

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Detalles Bibliográficos
Autores principales: Görlitz, Frederik, Kelly, Douglas J., Warren, Sean C., Alibhai, Dominic, West, Lucien, Kumar, Sunil, Alexandrov, Yuriy, Munro, Ian, Garcia, Edwin, McGinty, James, Talbot, Clifford, Serwa, Remigiusz A., Thinon, Emmanuelle, da Paola, Vincenzo, Murray, Edward J., Stuhmeier, Frank, Neil, Mark A. A., Tate, Edward W., Dunsby, Christopher, French, Paul M. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2017
Materias:
Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352269/
https://www.ncbi.nlm.nih.gov/pubmed/28190060
http://dx.doi.org/10.3791/55119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352269/
https://www.ncbi.nlm.nih.gov/pubmed/28190060
http://dx.doi.org/10.3791/55119

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