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Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision
We describe here a new approach to marker recycling, a controlled sequence of steps in which a genetic marker is selected and then lost. Marker recycling is important for genetic manipulation, because it allows a single selection marker to be used repeatedly. Our approach relies upon the ability of...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Microbiology
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352831/ https://www.ncbi.nlm.nih.gov/pubmed/28317025 http://dx.doi.org/10.1128/mSphere.00050-17 |